Effects of Some Mutagens and Priming Applications on M4 Generation in Bean Mutation Breeding

Abstract

In this study, it was carried out to determine some vegetative characteristics and yieldcharacteristics of 30 lines of M4 plants to which different mutagens were applied. As a mutagen, 120 Gygamma rays and 30 and 40 Mm Ethyl Methane Sulfonate (EMS), humidification and neem oil applicationswere among the trial subjects. Features such as pod length and width, pod weight, plant height, stemdiameter, number of pods, number of grains per plant and grain weight were investigated in mutant plantsthat have reached the M4 stage. The pod length of the examined lines is 13.50-16.29 cm, pod width 12.24-17.82 mm, pod weight 10.03-13.73 gr, plant height 46.00-74.17 cm, stem diameter 6.35-11.44 mm, numberof pods 21.27-45.43, number of grains 56.88-131.72 and the grain weight was found to be between 16.40-40.91 gr. It was observed that the pod length, which is one of the yield factors, increased by 214% comparedto the control group of H2E1, the weight of NH3E2 pod increased by 37% compared to the control group,the number of NHG1 grains increased by 232% compared to the control group, and the weight of NHG1grains increased by 246% compared to the control group. When the lines of all applications were examined,it was seen that humidification and humidification applications with neem oil reached the highest averagevalues, while the control group reached the lowest average values.