Cacti are the Cactaceae family and are a desert plants adapted to arid regions. Cacti known as succulent plants and store water in their leaves. They have a stems in arid region and soil conditions. They are used as landscaping, ornamental plants and potted plants. In addition to the vitamins and amino acids they contain, also rich in secondary metabolites. This study was conducted in tissue culture propagation of Opuntia microdasys, is named as ‘tavşan kulaklı’ cactus. Succulent stems, called pads, were used as genetic material. Opuntia microdasys were cultured on Murashige and Skoog (1962) medium (MS) with TDZ and BAP (0.5 mg L^-1; 1.0 mg L^-1; 2.0 mg L^-1; 3.0 mg L^-1). Best rejeneration were obtained with TDZ and BAP at 0.5 mg L^-1 as 3.5 for pad number. The media of 0.5 mg L^-1 TDZ was found to be the highest with 1.4 cm in terms of pad length. On the other hand, 0.5 mg L^-1 BAP medium had the highest mean for root number and root length, with 2.5 and 1.6 cm, respectively. It has been seen that the succulent variety Opuntia microdasys, not only use as an ornamental plant but also medicinal area. Opuntia microdasys has a potential to be evaluated under in vitro conditions. Therefore, this study can be developed using different nutrient media and growth regulation for shoot and root formation with following studies. So in vitro propagation for Opuntia microdasys can be an alternative to other production.